Location: Rothe-Geussenhainer-Haus (N45), third floor
Fluorescence: DAPI / GFP / RFP / Cy5 / Cy7
Software: Cell Sense Dimension v3
Live cell imaging: Environmental chamber with temperature, humidity and CO2 control
| Microscope | Olympus IX-83 |
| Objectives | 4x UPLFLN PH NA: 0.13 WD (mm): 17 Pixel size in image (µm): 1.622 20x UCPLFLN PH NA: 0.7 WD (mm): 1.3 Pixel size in image (µm): 0.338 100x UPLXAPO O NA: 1.45 WD (mm): 0.13 Pixel size in image (µm): 0.064 |
| DIC prisms | Condenser:
Slider:
|
Cameras | Monochrome: Hamamatsu Flash 4.0 LTPlus Color: SC50 |
| Fluorescence filters fluo-turret | Filterset no. (em.-color; dye): excitation | beamsplitter | emission
|
| Fluorescence excitation (LED) | Excitation (nm)
|
UV-VIS illumination | CoolLED pE-4000, 16 LEDs for excitation in the range 365-770 nm |
Halogen lamp | 100W 12V, standard housing |
Acquisition software | OlympusCell Sense Dimension V 3.1 |
Incubation unit | Pecon cellVIVO closed large chamber for temp./CO2/humidity |
| Motorized XY-stage | Märzhäuser Tango |
| Hardware autofocus | ZDC2 |
| Passively damped optical board | Thorlabs |
Gallery
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