UKE Microscopy Imaging Facility (UMIF)

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

MINFLUX SARS-CoV2

MINFLUX microscopy made the visualization of SARS-CoV2 virus molecules possible.

(1) Confocal, (2) MINFLUX, (3) MINFLUX superimposed with EM,

(4) schematic overlay with SARS-CoV2 model

CONFOCAL MICROSCOPY III

GFAP (for astrocytes) / Iba-1 (microglia) / Hoechst (nucleus).

Author: Prof Saez

CONFOCAL MICROSCOPY I

Optical nerve

Tiled scan

CONFOCAL MICROSCOPY II

Heart tissue section

SUPER-RESOLUTION IMAGE OF DETYROSINATED TUBULIN

Detyrosinated tubulin in neurons.

left = confocal, right = STED

Super-resolution 2D dSTORM image of nuclear pore complex (NPC)

NPC with Abberior FLUX640

STRUCTURED ILLUMINATION MICROSCOPY (SIM)

Multicolor labeling of an eukaryotic cell.

red =actin, green = focal adhesions, blue = DAPI

COMBINED SD-TIRF IMAGING OF CELLULAR VESICLES

3D reconstruction of CLN3-vesicles and lysosomes in HeLa cells.

green = CLN3-GFP, red = lysosomes

SUPER-RESOLUTION RADIAL FLUORESCENCE MICROSCOPY (SRRF)

Multi-color HeLa cells

green = F-Actin, red = alpha-Tubulin, gray = TOMM-20

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Latest news and events

UMIF Lecture 2024

UMIF together with UKE scientific community is organizing the second UKE  “microscopy from single molecule to living embryo”. This course is the follow up of the first UKE “light microscopy from single molecules to living embryo”. As you can see the word “light” has been removed because this year also Electron microscopy for biomedical application will be covered. Other added topics are: sample preparation for basic advanced and super resolution microscopy. This course will run between November and January and will consist of 17 lectures for a total of 34 hours (that will provide you didactic credits). The course is made for everyone who want to learn about microscopy from the basic concepts to the more advanced super resolution techniques.  In this course will be stressed out how important is the design of a light microscopy experiments and the huge impact that sample preparation has on their successful outcome. Last but not least some basic notion of image analysis and quantification will also be offered. If you are interested in widen your horizon including microscopy in your knowledge portfolio this course is made for you, especially if you would like to get inspired regarding which images techniques are available at UKE and how they could impact your research projects.

Here is the detailed program of the course

Here is the course timeline

Here is the link for the registration

 

EVIDENT Open House, Hamburg, July 9–11, 2024

Dear Users,

Evident, one of the most prominent microscopy company, is opening the doors of the their main quarter in Hamburg.

When?  9-11.07

You will find a comfortable atmosphere and the opportunity to test the newest products as for example the Evident FV4000 the first fully quantitative confocal microscope on the market.

Obviously there is not only this incredible machine to see but much more....  please see the flyer

Open House Evident

We are welcome to book our visiting slot and to diffuse this invitation to everyone might be interested

 

best wishes

Antonio Virgilio Failla

OPTISCAN ViewnVivo Demonstration

Dear Users,

Optiscan is introducing us with a practical demonstration about how the smallest ever intravital confocal microscope works.

This microscope can be placed close to any living tissue or organ and take detailed high resolution pictures.

The demonstration will take place on Tuesday 02.07

More information you will find in the flyer.

OPTISCAN ViewnVivo Demonstration

I found this imaging tool extremely interesting for intravital scientists and for clinical application.

UMIF it is still not well connected with clinic researcher therefore I would ask you any of you that has this connection with clinical and diagnosis lab to spread this news. Sample of UKE would be also welcome to tested, so please let me know if there are potential samples  and, depending on their nature they will be either imagined on the demonstration site or Optiscan will come to our labs.

Please if you intend to participate give me your feedback by email and please help me to diffuse this information because I know that many potential users will be not reached by UMIF news letter and  we all share the mission to make UKE  a world wide top research institute.

Looking forward to your participation and feedback

best wishes

Antonio Virgilio Failla

User 1000 prize celebration

Dear Users,

Thank you so much for joining the celebration for 1000 UMIF users which was held on 01.03.2024.

Despite the strike on the same day, there are over 60 people joined the party. The 1000# user got a small prize from the UMIF team and afterwards we had our international buffet, good music and relaxing time.

I hope you all enjoyed the event and here I shared some images from the night. Thank you for being a part of it and looking forward to seeing you next time!

 UMIF 1000 poster

umif1000 party 1

umif1000 party 2

umif1000 party 6

umif1000 party 4

umif1000 party 3

 

best wishes

Antonio Virgilio Failla

Abbelight DNA-PAINT workshop

Dear Users

from April 16 - 18, 2024, there will be a super interesting workshop from one of the world leading microscope manufacturer Abbelight at CSSB with a focus on DNA-PAINT.
DNA-PAINT is a powerful super-resolution light microscopy technique that might be rather easy to use and allowed multiplex imaging. This approach achieves a resolution of a few ten nm (https://www.abbelight.com/dna-paint/).
Please be aware that UMIF has a super resolution microscope of Abbelight!!!!!

If you are interested in this technique, please join an online prep meeting on March 14, 2024 at 16:00. During the prep meeting the technique will be explained and we can discuss experiments that you might want to carry out with your own samples. Abbelight can then provide DNA-PAINT staining reagents that you can use beforehand and then measure your samples at the workshop.

If you want to join the workshop and/or the online prep meeting, please send an email to This email address is being protected from spambots. You need JavaScript enabled to view it. so that we can assemble a list of interested people.
Please diffuse this information to anyone might be interested, this is a unique chance to use.

Best wishes
Antonio Virgilio Failla

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