UKE Microscopy Imaging Facility (UMIF)

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

MINFLUX SARS-CoV2

MINFLUX microscopy made the visualization of SARS-CoV2 virus molecules possible.

(1) Confocal, (2) MINFLUX, (3) MINFLUX superimposed with EM,

(4) schematic overlay with SARS-CoV2 model

CONFOCAL MICROSCOPY III

GFAP (for astrocytes) / Iba-1 (microglia) / Hoechst (nucleus).

Author: Prof Saez

CONFOCAL MICROSCOPY I

Optical nerve

Tiled scan

CONFOCAL MICROSCOPY II

Heart tissue section

SUPER-RESOLUTION IMAGE OF DETYROSINATED TUBULIN

Detyrosinated tubulin in neurons.

left = confocal, right = STED

Super-resolution 2D dSTORM image of nuclear pore complex (NPC)

NPC with Abberior FLUX640

STRUCTURED ILLUMINATION MICROSCOPY (SIM)

Multicolor labeling of an eukaryotic cell.

red =actin, green = focal adhesions, blue = DAPI

COMBINED SD-TIRF IMAGING OF CELLULAR VESICLES

3D reconstruction of CLN3-vesicles and lysosomes in HeLa cells.

green = CLN3-GFP, red = lysosomes

SUPER-RESOLUTION RADIAL FLUORESCENCE MICROSCOPY (SRRF)

Multi-color HeLa cells

green = F-Actin, red = alpha-Tubulin, gray = TOMM-20

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Latest news and events

UMIF celebrates its 15th anniversary with a fresh new logo

Dear UMIF family, 

As part of the 15th UMIF birthday, we've upgraded our logo. We decided on a modern look with a pinch of history. Below a little reveal clip. Stay tuned for more action! Tschüss:)

MI-SIM Super-Resolution Workshop

Dear users,

I would like to let you know, that following up the very interesting webinar, CRS Biotech will bring their super-resolution live-cell imaging platform to UKE.

We would love you to challenge this system with your samples. It claims to achieve 60 nm optical resolution in live-cell imaging condition.

There are still some slot free and it would be a pity to not use them all.

Please reserve your demo slot by using the QR code present in the attached flyer and do not hesitate to contact me if you have questions about the sample preparation.

Please diffuse this information in your network and inform anyone who might be interested. You are all warmly invited <3

 

 

 

Super resolution light nanoscopy at the single molecule level workshop (09-13.02.2026)

The UKE Microscopy Imaging Facility (UMIF), Abberior Instruments, Abberior Dyes and Abbelight invite you to:


Get inspired by cutting-edge applications of super resolution light nanoscopy in biomedical research.
Learn how to design samples and the labels available for super-resolution light nanoscopy.


Get familiar with the most advanced super-resolution techniques: STED, camera-based SMLM and MINFLUX nanoscopy.


Go to the microscopes for hands-on sessions!


Book a new experience with the MIRAVA Polyscope, our special guest for the entire workshop.

Some news and Reminder on Urgent Facility movement

Dear all

I forward with this email the text I already written in a previous email plus some additional information

1)  please remind to send us the data of the paper you publish where you acknowledge either the UMIF or DFG grant for the microscope. For this scope use the following link:

https://www.umif.de/contact-us/publication-report

2) the movement started together with some technical problems at the microscopes. Nothing tragic but it will require some time before the moved microscopes will be operative again.

3) when you do your re-registration process (see attached information) please make sure that the data you insert is correct as for example the user name otherwise the re-registration process might fail.

4) In these days we are a bit emotional. Working in the facility we are used to see wonderful people coming and living. When nice people is leaving it is always  sad but also nice because it means a new life chapter is open for them. Few days ago we got to know that one of our most beloved users, due to a life accident, left us without having the opportunity to say good bye. We will always remind his sunny smile and kindness also when in front of our criticisms. Good bye Jyothish !!!!

Best wishes

Antonio Virgilio Failla

Cluster nanoarchitecture and structural diversity of PIEZO1 at rest and during activation in intact cells | Science Advances

Cluster nanoarchitecture and structural diversity of PIEZO1 at rest and during activation in intact cells | Science Advances

https://www.science.org/doi/10.1126/sciadv.ady8052

would like to say: once more the UKE scientists demonstrate that MINFLUX microscopy can push up the research at international prominent level.

This is not a proof of principle paper but a high impact scientific work. I share this with you because at UKE we take pride to be in between the firsts that have understood the potential of MINFLUX microscopy. The important point is to approach novel light microscopy techniques without positive or negative prejudices but with open mind and curiosity. If you are open minded and curious please try, test and discover the light microscopy approach that can boost your research!!!!

 

best wishes

Antonio Virgilio Failla

 

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