UKE Microscopy Imaging Facility (UMIF)

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

MINFLUX SARS-CoV2

MINFLUX microscopy made the visualization of SARS-CoV2 virus molecules possible.

(1) Confocal, (2) MINFLUX, (3) MINFLUX superimposed with EM,

(4) schematic overlay with SARS-CoV2 model

CONFOCAL MICROSCOPY III

GFAP (for astrocytes) / Iba-1 (microglia) / Hoechst (nucleus).

Author: Prof Saez

CONFOCAL MICROSCOPY I

Optical nerve

Tiled scan

CONFOCAL MICROSCOPY II

Heart tissue section

SUPER-RESOLUTION IMAGE OF DETYROSINATED TUBULIN

Detyrosinated tubulin in neurons.

left = confocal, right = STED

Super-resolution 3D dSTORM image of microtubules

Alpha-tubulin in HeLa cells.

Color-coded z-distribution.

STRUCTURED ILLUMINATION MICROSCOPY (SIM)

Multicolor labeling of an eukaryotic cell.

red =actin, green = focal adhesions, blue = DAPI

COMBINED SD-TIRF IMAGING OF CELLULAR VESICLES

3D reconstruction of CLN3-vesicles and lysosomes in HeLa cells.

green = CLN3-GFP, red = lysosomes

SUPER-RESOLUTION RADIAL FLUORESCENCE MICROSCOPY (SRRF)

Multi-color HeLa cells

green = F-Actin, red = alpha-Tubulin, gray = TOMM-20

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User regulations, booking rules, charging scheme, course material, software, protocols and more

Latest news and events

LIV-Symposium Viruses, Imaging & Materials

Dear users,

A very Interesting symposium with exceptional guests will take place  at LIV (for more information please have a look at the flyer). Registration can be accomplished following the indication on the flyer. Please diffuse this information to every one who might be interested.

 

 

Best wishes

Antonio Virgilio Failla

Compact and powerful temperature control device from Interherence Gmbh

Dear users,

Interherence GmbH is a spin-off of the Max Planck Institute for the Science of Light in Erlangen, Germany. Their product, VAHEAT, is a very interesting compact and powerful device to control the temperature of your sample during live imaging. This device can be customized on any microscope allowing the opportunity to have live cell imaging also in microscopes that does not have a proper incubator (for more information please have a look at the flyer ). Please diffuse this information to anyone who might be interested but not belonging to the imaging facility. If you express your interest in testing the device by emailing me (This email address is being protected from spambots. You need JavaScript enabled to view it.), we might have the chance to have it here for demo.

VAHEAT Flyer 

 

Best wishes

Antonio Virgilio Failla

Compact setup for in vivo imaging made by Optiscan

Dear users,

Depending on your interest we might be entitled to test a very important compact setup for in vivo imaging made by Optiscan (for more information please have a look at the  flyer). Please consider this setup might be useful also to colleagues working in the hospital and that are not using the imaging facility. Please do not hesitate to diffuse to them this information.

 

If interest from you arises please contact me (This email address is being protected from spambots. You need JavaScript enabled to view it.) and there might be the chance to test this very interesting device.

 

 

Best wishes

Antonio Virgilio Failla

User Number 1000 Prize and Celebration

Dear users,

Thanks to all of you the light microscopy facility has achieved incredible milestones, personal as well as scientific.

We are aiming to celebrate with all of you one of those: our user number 1000.

The user number 1000 prize will be given on Friday 01.03.2024 in seminar room 14 at 5pm.

The user number 1000 prize will give us the chance to organize a buffet with Italian Chinese, German and maybe also your favorite food.....

In order to organize food and drinks If you wish to participate (and you are all super warmly invited to do so) please register on this link:

https://docs.google.com/spreadsheets/d/1bIfcwO1KOWy__x2AMLd5_G0pjNjzVnJhm0tXA_WxvYo/edit?usp=sharing

As you can see from the registration form if you wish you can join us preparing some food but this is a free willing option

We are looking forward to share with you good food and mood

 

best wishes

Antonio Virgilio Failla

Achievements at UMIF: a cover image has been awarded to Sven Hey (Ag Linder)

Sven Hey (Ag Linder) has recently published “KIF16B drives MT1-MMP recycling in macrophages and promotes co-invasion of cancer cells” in “Life Science Alliance” (DOI: 10.26508/lsa.202302158). Beside that we are all warmly invited to read this very interesting work, we take much pride to inform you that one of the images associated to this manuscript has been awarded to be the cover image for the entire issue!!!

Congratulation to Sven that expressed his scientific and imaging talent using UMIF microscopes

Cover Life Science Alliance 2023

About the image: Spheroid of H1299 carcinoma cells expressing GFP (green), embedded in collagen I matrix, together with primary human macrophages; F-actin of both cell types stained by Alexa568-phalloidin (red), nuclei by DAPI (blue); maximum projection of confocal z-stack.

The cover image was acquired by using UMIF´s SP8 microscope.

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