UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

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Sysmex Pannoramic MIDI II

Sysmex Pannoramic MIDI II

 

Location: room 02.029, second floor

Fluorescence: DAPI / FITC/ TRITC / Cy5

Software: Sysmex Pannoramic Scanner v3.0.6

 

Microscope Sysmex Pannoramic MIDI II
Objectives

20x Zeiss Plan-Apochromat

NA: 0.8

WD (mm): 0.55


40x Zeiss Plan-Apochromat Korr

NA: 0.95

WD (mm): 0.25

Coverslip (mm): 0.13-0.21

Camera 1x Teledyne FLIR Grasshopper 3 GS3-U3-51S5M (CMOS)
Fluorescence filters in microscope stand

Filtersystem (em.-color, dye): excitation | beamsplitter | emission

  • Blue (DAPI, Hoechst):

  • Green (FITC, AF488, GFP):

  • Red (TRITC, AF568, RFP):

  • Far-red (Cy5, AF633)
UV-lamp Lumencor SOLA light engine, 4 solid state illumination sources for excitation in the range 380-660 nm
LED lamp White light LED
Software Sysmex Pannoramic Scanner v3.0.6

Applications

Widefield, Slide scanner
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