UMIF

5x EC Plan-Neofluar

NA: 0.16

WD (mm): 18.5

Pixel size in image (µm): 1.290

10x A-Plan Ph1

NA: 0.25

WD (mm): 4.5

Pixel size in image (µm): 0.645

20x LD Plan-Neofluar Korr Ph2

NA: 0.4

WD (mm): 7.9 @ cover glass 0.75

Coverslip thickness: 0-1.5

Pixel size in image (µm): 0.323

20x Plan-Apochromat

NA: 0.8

WD (mm): 0.55

Pixel size in image (µm): 0.323

40x EC Plan-Apochromat Oil

NA: 1.4

WD (mm): 0.13

Pixel size in image (µm): 0.161

63x Plan-Apochromat Oil DIC

NA: 1.4

WD (mm): 0.19

Pixel size in image (µm): 0.102

40x EC Plan-Neofluar

NA: 0.75

WD (mm): 0.71

Pixel size in image (µm): 0.161

63x EC Plan-Neofluar Oil

NA: 1.25

WD (mm): 0.10

Pixel size in image (µm): 0.102

Condenser:

• DIC II and DIC III

Sliders:

• A: 20x
• 63x ‘standard’: Oil NA 1.25 and water NA 1.2 (both with DIC III)
• 63x ‘high contrast’: Oil NA 1.25 and water NA 1.2 (both with DIC II)

• CCD AxioCam HRm 1.4MP monochrome
  • Gain: 0.5 e^-/ADU
• CCD AxioCam MRc 1.4MP color

Colibri 7, 5 LEDs for excitation with 385/475/555/590/630 nm

Filtersystem (em.-color, dye): excitation | beamsplitter | emission

• 96 HE (blue; DAPI):
  BP 390/40 | FT 420 | BP 450/40

• 38 HE (green; AF488, GFP):
  BP 470/40 | FT 495 | BP 525/50

• 43 HE (red; AF568, mCherry):
  BP 550/25 | FT 570 | BP 605/70

• 50 (far-red; AF633, CY5):
  BP 640/30 | FT 660 | BP 690/50

• 32 (optional; far-red; AF680, CY5.5):
  BP 665/45 | FT 695 | BP 725/50