UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

Zeiss ApoTome

Zeiss ApoTome

 

Location: room 02.053, second floor

Fluorescence: DAPI / GFP / RFP / Far-Red

Software: ZEN 3.4

Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

 

 

Microscope Zeiss Axio Observer
Objectives (installed)

5x EC Plan-Neofluar

NA: 0.16

WD (mm): 18.5

Pixel size in image (µm): 1.290


10x A-Plan Ph1

NA: 0.25

WD (mm): 4.5

Pixel size in image (µm): 0.645


20x LD Plan-Neofluar Korr Ph2

NA: 0.4

WD (mm): 7.9 – 0.75

Pixel size in image (µm): 0.323


20x Plan-Apochromat

NA: 0.8

WD (mm): 0.55

Pixel size in image (µm): 0.323


40x EC Plan-Apochromat Oil

NA: 1.4

WD (mm): 0.13

Pixel size in image (µm): 0.161


63x Plan-Apochromat Oil DIC

NA: 1.4

WD (mm): 0.19

Pixel size in image (µm): 0.102

Objectives (spare/'Ersatz')

40x EC Plan-Neofluar

NA: 0.75

WD (mm): 0.71

Pixel size in image (µm): 0.161


63x EC Plan-Neofluar Oil

NA: 1.25

WD (mm): 0.10

Pixel size in image (µm): 0.102

DIC-Prisms

Condenser:

  • DIC II and DIC III

Sliders:

  • A: 20x
  • 63x ‘standard’: Oil NA 1.25 and water NA 1.2 (both with DIC III)
  • 63x ‘high contrast’: Oil NA 1.25 and water NA 1.2 (both with DIC II)
Cameras
  • CCD AxioCam HRm 1.4MP monochrome
    • Gain: 0.5 e-/ADU
  • CCD AxioCam MRc 1.4MP color
UV-Lamp

Colibri 7, 5 LEDs for excitation with 385/475/555/590/630 nm

Fluorescence filters in microscope stand

Filtersystem (em.-color, dye): excitation | beamsplitter | emission

  • 96 HE (blue; DAPI):
    BP 390/40 | FT 420 | BP 450/40

  • 38 HE (green; AF488, GFP):
    BP 470/40 | FT 495 | BP 525/50

  • 43 HE (red; AF568, mCherry):
    BP 550/25 | FT 570 | BP 605/70

  • 50 (far-red; AF633, CY5):
    BP 640/30 | FT 660 | BP 690/50

  • 32 (optional; far-red; AF680, CY5.5):
    BP 665/45 | FT 695 | BP 725/50

Transillumination LED
Software Zeiss ZEN Pro 3.4
Incubation unit Pecon XLS1
ApoTome 3 for structured Illumination  
Anti-vibrational plate  Newport

Applications

Live imaging, Structured illumination, Widefield
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