UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

Leica TCS SP5

Location: room 02.046, second floor

Laser lines: Diode: 405 nm; Multi-Ar 458 nm/476 nm/488 nm/496 nm/514 nm; DPSS 561 nm; HeNe 633 nm

Software: Leica LAS

Live cell imaging: Add-on environmental perfusion chamber with temperature, humidity and CO2 control

Microscope	Leica DMI 6000
Objectives	10x HCPL FLUOTAR NA: 0.30 WD (=working distance; mm): 11.0 20x HC PL APO CS IMM/CORR NA: 0.70 WD (mm): 0.59 (W), =.17 (oil) 25x WATER HC FLUOTAR L VISIR NA: 0.95 WD (mm): 2.5 40x HC PL APO CS2 Oil NA: 1.30 WD (mm): 0.24 63x HCX PL APO Lbd. Bl. Oil NA: 1.4-0.60 WD (mm): 0.1 63x WATER HCX PL APO CS NA: 1.2 WD (mm): 0.22
Detectors	3x HyD, 1x PMT, 1x Trans-PMT
Laser lines (nm)	Diode: 405 Multi-Ar: 458 / 476 / 488 / 496 / 514 DPSS: 561 HeNe: 633
Pinhole size	variable
Spectral separation	AOBS® + spectral detectors
Software	Leica LAS AF
Filters for fluorescence	Filtersystem (em.-color, dye): excitation \| beamsplitter \| emission L5 ET (green; AF488, GFP): BP 480/40 \| FP 505 \| em. BP 527/30 N3 ET (red; AF568, mCherry): BP 546/12 \| FP 565 \| em. 600/40 A (blue; DAPI): BP 340-380 \| FP \| LP 425 I 3 (green; AF488, GFP): BP 450-490 \| FP \| LP 515 N 2.1 (red; AF568, mCherry): BP 515-560 \| FP \| LP 590
UV-lamp	EL 6000 120W (LQHXP 120 LEJ)
Halogen lamp	100W 12V
CO2 controller	BRICK/Ludin
Temperature controller	Ludin Cube
Piezo focus drive	SuperZ Galvo type H (travel range: 500 µm; reproducibility: 40 nm)
Tandem-Scanner	Variable: 512x512 @ 5 fps; max. 8192x8192 px; max. 2800 lines/s; 1x-32x zoom Resonant: 512x512 @ 25 fps; max. 1024x1024 px; max. 16.000 lines/s; 1.7x-32x zoom
Large incubation chamber	Ludin
Mini-incubation chamber for PiezoZ	Ludin
Actively damped optical table	Newport
Silencer for Multi-Ar laser

Panel Footer

Confocal, FCS/FCCS, Live imaging, FRET, FRAP, Photoactivation/-conversion, Spectral unmixing