UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

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Abbelight SAFe360

Abbelight SAFe360

DFG code: INST 152/933-1

 

Location: Campus Forschung (N27), room 02.046, second floor

Laser lines: 640nm

Software: Abbelight NEO

 

Microscope Olympus IX-83
Objectives

100x UPlan Apo HR TIRF Oil

NA: 1.50

WD (mm): 0.11 (CS: 0.13-0.19 @ 37 °C)

Pixel size in image (µm): 0.96

Cameras

2x Hamamatsu ORCA-fusion C14440

Laser lines (nm)

Solid-state: 

  • 640 (1500mW?)
Acquisition software Abbelight Neo acquisition v.?
Analysis software

Abbelight NEO analysis v.?

Image scanning technology Adaptative Scanning for Tuneable Excitation Regions (ASTER)
TIRF light path  TIRF translational stage for epi, HiLO & TIRF excitation
2D SMLM Abbelight SAFe 180/360, simultaneous dual-color, spectral demixing
3D SMLM extension Abbelight SAFe 360 with astigmatism lens
Hardware autofocus

IX3-ZDC

Actively damped optical table Newport

Applications

Superresolution, 2D/3D Dual color SMLM
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