UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

.

User 1000 animation

Welcome...

User #1000

Zeiss LSM980

Zeiss LSM980

DFG code: INST 152/952-1 FUGG

 

Location: Campus Forschung (N27), room 02.053, second floor

Laser lines: 405/445/514/488/561/594/639/730nm

Software: ZEN

Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

 

Microscope Zeiss Axio Observer 7 SP with LSM 980 and Airyscan 2 (inverted)
Objectives

5x Plan-APOCHROMAT

NA: 0.16

WD (=working distance; mm): 12.1


10x Plan-APOCHROMAT

NA: 0.45

WD (=working distance; mm): 2.1


20x Plan-APOCHROMAT

NA: 0.8

WD (mm): 0.55


40x C-Apochromat W Corr

Coverslip (mm): 0.13-0.20

NA: 1.2

WD (mm): 0.28 (@0.17mm)


63x Plan-APOCHROMAT Oil DIC

NA: 1.40

WD (mm): 0.19

DIC-prisms 10x, 20x, 40x, 63x
Detectors

Confocal unit:

- 32x GaAsP

- 2x PMT (UV&NIR)

- 1x GaAs NIR

- 1x Trans-PMT

- Airyscan 2

Camera Zeiss Axiocam 305 mono R2
Laser lines (nm)

Diodes: 405 (14mW) / 445 (7.5mW) / 514 (10mW) / 488 (10mW) / 639 (7.5mW) / 730 (9.5mW)

DPSS: 561 (10mW), 594 (2.5mW)

Pinhole size variable
Software Zeiss ZEN Blue 3.8
Filters for fluorescence

For 32+2 channel spectral detection: tunable between 380-900nm

For eyepiece:

Filter system (em.-color, dye): excitation | beamsplitter | emission

  • 49 (blue; AF405, DAPI):
    BP 365 | FT 395 | BP 445/50
  • 38 (green; AF488, GFP):
    BP 470/40 | FT 495 | BP 525/50
  • 43 (red; AF568, mCherry):
    BP 550/25 | FT 570 | BP 605/70

For Airyscan:

  1. BP 420-480 + BP 495-550
  2. BP 420-480 + BP 570-630
  3. BP 420-500 + LP 605
  4. BP 465-505 + BP 525-585
  5. BP 495-550 + BP 570-630
  6. BP 495-560 + LP 660
  7. BP 570-620 + LP 660
UV-VIS illumination Zeiss Colibri 5 (385, 475, 555, 630 nm)
Halogen lamp 100W 12V
XY-Scanner
  • Variable
  • Multiplex: 4 spots or 8 spots with Airyscan up to 47 fps @ 512x512
Z-Scanner PiezoZ, 500µm travel
Actively damped optical table Newport Vision Iso Station
Incubation

Chamber: XL Multi S2 Dark Premium

Heating Unit: XL S2

Temperature/gas control: ibidi

 

Applications

Confocal, Superresolution, FCS/FCCS, Live imaging, FRET, FRAP, Photoactivation/-conversion, Spectral unmixing, Airyscan
© 2023 All Rights Reserved. Designed By JoomShaper. Powered by Joomla.