UMIF
UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)
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User #1000
PhaseView LS imaging platform
Location: Campus Forschung (N27), room 02.029, second floor
Laser lines: 405nm/488nm/561nm/638nm/730nm
Software: QtSPIM
| Microscope | PhaseView |
| Illumination objectives (2 each) |
Mitutoyo M Plan Apo 2x NA: 0.055 WD (mm): 34 Sheet thickness@488nm [µm]: min. 9 Mitutoyo M Plan Apo 5x NA: 0.14 WD (mm): 34 Sheet thickness@488nm [µm]: min. 6 Mitutoyo M Plan Apo 10x NA: 0.28 WD (mm): 34 Sheet thickness@488nm [µm]: min. 1.5 |
| Detection objectives |
Olympus XLFluor 4x NA: 0.28 WD (mm): 28.8-30 Olympus 10x UMPlanFL N water NA: 0.30 WD (mm): 3.5 Olympus XLPlan N 10x SVMP NA: 0.60 WD (mm): 8 Correction collar for Rf: 1.33-1.52 Olympus UMPlanFL N 20x water NA: 0.5 WD (mm): 3.5 Olympus XLSLPlan N 25x GMP NA: 0.95 WD (mm): 8 Correction collar for Rf: 1.41-1.52 |
| Camera |
Hamamatsu Flash4 v3 C13440-20CU, 6.5µm pixel size |
| Laser lines (nm) |
Solid-state: 405 / 488 / 561 / 638 / 730 |
| Fluorescence emission filters |
Filtersystem (em.-color, dye): emission [nm]
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| Transmitted light lamp | LED |
| Software | PhaseView QtSPIM |
| Motorized XYZ control | Physics Instruments |
| Focus control | Focus sweeping, dynamic focus |