UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

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PhaseView LS imaging platform

PhaseView LS imaging platform

 

Location: Campus Forschung (N27), room 02.029, second floor

Laser lines: 405nm/488nm/561nm/638nm/730nm

Software: QtSPIM

 

Microscope PhaseView
Illumination objectives (2 each)

Mitutoyo M Plan Apo 2x

NA: 0.055

WD (mm): 34

Sheet thickness@488nm [µm]: min. 9


Mitutoyo M Plan Apo 5x

NA: 0.14

WD (mm): 34

Sheet thickness@488nm [µm]: min. 6


Mitutoyo M Plan Apo 10x

NA: 0.28

WD (mm): 34

Sheet thickness@488nm [µm]: min. 1.5

Detection objectives

Olympus XLFluor 4x

NA: 0.28

WD (mm): 28.8-30


Olympus 10x UMPlanFL N water

NA: 0.30

WD (mm): 3.5


Olympus XLPlan N 10x SVMP

NA: 0.60

WD (mm): 8

Correction collar for Rf: 1.33-1.52


Olympus UMPlanFL N 20x water

NA: 0.5

WD (mm): 3.5


Olympus XLSLPlan N 25x GMP

NA: 0.95

WD (mm): 8

Correction collar for Rf: 1.41-1.52

Camera

Hamamatsu Flash4 v3 C13440-20CU, 6.5µm pixel size

Laser lines (nm)

Solid-state: 405 / 488 / 561 / 638 / 730

Fluorescence emission filters

Filtersystem (em.-color, dye): emission [nm]

  • Blue (DAPI):
    BP ???-????

  • Green (AF488, GFP):
    BP ???-????

  • Red (AF568, mCherry):
    BP ???-????

  • Far-red (AF633, Cy5):
    BP ???-????
  • Near-IR (AF750, Cy7):
    BP ???-????
Transmitted light lamp LED
Software PhaseView QtSPIM
Motorized XYZ control Physics Instruments 
Focus control Focus sweeping, dynamic focus
   

Applications

Light Sheet
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