UMIF
UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)
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User #1000
Evident SD
DFG code: INST 152/933-1
Location: Campus Forschung (N27), room 02.029, second floor
Laser lines: 405nm/488nm/561nm/640nm
Software: Cell Sense
Live cell Imaging: Add-on environmental chamber with temperature, humidity and CO2 control
| Microscope | Olympus IX-83 |
| Objectives |
4x UPlanXApo NA: 0.16 WD (mm): 13 Pixel size in image (µm): 1.625; with 3.2x magnification changer: 0.508 10x UPlanXApo NA: 0.40 WD (mm): 3.1 Pixel size in image (µm): 0.65; with 3.2x magnification changer: 0.203 20x UPlanXApo NA: 0.8 WD (mm): 0.6 Pixel size in image (µm): 0.325; with 3.2x magnification changer: 0.1016 40x UPlanXApo (optionally available) Coverglass (mm): 0.11-0.23 NA: 0.95 WD (mm): 0.18 Pixel size in image (µm): 0.1625; with 3.2x magnification changer: 0.051 100x UPlanApo HR Coverglass (mm): 0.13-0.19 @ 23 °C & 37 °C NA: 1.5 WD (mm): 0.12 Pixel size in image (µm): 0.065; with 3.2x magnification changer: 0.02 100x UPlanSApo (Silicon oil) Coverglass (mm): 0.13-0.19 @ 23 °C & 37 °C NA: 1.35 WD (mm): 0.2 Pixel size in image (µm): 0.065; with 3.2x magnification changer: 0.02 |
| Cameras |
Orca Fusion C14440 (back-illuminated sCMOS, 6.5µm pixel-size, 2304x2304 pixels) |
| Laser lines (nm) |
Solid-state: 405 / 488 / 561 / 640 |
| Spinning Disk unit |
Yokogawa CSU W-1 |
| Emission filters spinning disk (for laser-related fluorescence) |
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| Dichroic in spinning disk unit |
405/488/561/640 (used with the respective channels) |
| Fluorescence filters in microscope stand |
Filtersystem (em.-color, dye): excitation | beamsplitter | emission
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| Fluorescence light source | CoolLED pE-300 ultra |
| Transmitted light lamp | Olympus LED |
| Software | Cell Sense |
| Incubation unit | PECON cell vivo |
| Piezo focus drive | Mad City Labs Nano-Z |
| Motorized XY stage |
Olympus |
| Auto-focus | Olympus TruFocus zero drift compensation |
| Actively damped optical table | Newport Vision IsoStation |