UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

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Olympus FV3000

Olympus FV3000

DFG code: INST 152/933-1

 

Location: Campus Forschung (N27), room 02.046, second floor

Laser lines: solid state 405, 445, 514, 488, 561, 594, 640 nm

Software: Olympus FV-3000

Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

 

 

Microscope Olympus IX83
Objectives

2x PlanApo N

NA: 0.08

WD (=working distance; mm): 6.2


4x UPlanXApo (optionally available)

NA: 0.16

WD (mm): 13


10x UPlanXApo

NA: 0.40

WD (mm): 3.1


20x UPlanXApo

NA: 0.8

WD (mm): 0.6


30x UPlanSApo Sil

Coverglass (mm): 0.13-0.19

NA: 1.05

WD (mm): 0.8


40x UPlanXApo (optionally available)

Coverglass (mm): 0.11-0.23

NA: 0.95

WD (mm): 0.18


60x UPlanSApo Sil (optionally available)

Coverglass (mm): 0.15-0.19

NA: 1.3

WD (mm): 0.3


60x PlanApo N SC Oil

NA: 1.4

WD (mm): 0.12


60x UPlanApo HR Oil

Coverglass (mm): 0.13-0.19

NA: 1.50

WD (mm): 0.11

Detectors High Sensitivity Spectral Detector with 4x GaAsP PMT, 1x Trans PMT
Laser lines (nm) Solid state: 405 / 445 / 488 / 514 / 561 / 594 / 640
Pinhole size variable
Spectral separation

Motorized Volume Phase Holographic (VPH) transmission diffraction grating

Hybrid-Scanner
  • Galvo: 512x512 @ 1 fps (16 fps interlaced mode); Tornado scanning for fast ROI scanning
  • Resonant: 512x512 @ 30 fps; max. speed: 512x32 @ 438 fps
Software Olympus FV-3000
Filters for fluorescence

Filtersystem (em.-color, dye): excitation | emission

  • Triple filter cube DAPI/FITC/TRITC (Chroma 69000):
    • DAPI: 392 - 416 nm | 440 - 462 nm
      FITC: 473 - 495 nm | 543 - 561 nm
      TRITC: 507 - 533 nm | 574 - 618 nm
UV-VIS illumination CoolLED pE-300white
Transmitted light LED (IX3-LHLEDC)
Contrast enhancement Differentiel interference contrast (DIC)
CO2 controller Cell vivo CB1G
Temperature controller Cell vivo CBT with Pecon heating unit 2000
Large incubation chamber Cell vivo/Pecon
Mini-incubation chamber for PiezoZ Cell vivo/Pecon
Actively damped optical table Newport Vision IsoStation

Applications

Confocal, Live imaging, FRET, Photoactivation/-conversion, Spectral unmixing
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