UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

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User #1000

Olympus FV3000

DFG code: INST 152/933-1

Location: Campus Forschung (N27), room 02.046, second floor

Laser lines: solid state 405, 445, 514, 488, 561, 594, 640 nm

Software: Olympus FV-3000

Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

Click here for a detailed description of the components

Microscope	Olympus IX83
Objectives	2x PlanApo N NA: 0.08 WD (=working distance; mm): 6.2 4x UPlanXApo (optionally available) NA: 0.16 WD (mm): 13 10x UPlanXApo NA: 0.40 WD (mm): 3.1 20x UPlanXApo NA: 0.8 WD (mm): 0.6 30x UPlanSApo Sil Coverglass (mm): 0.13-0.19 NA: 1.05 WD (mm): 0.8 40x UPlanXApo (optionally available) Coverglass (mm): 0.11-0.23 NA: 0.95 WD (mm): 0.18 60x UPlanSApo Sil (optionally available) Coverglass (mm): 0.15-0.19 NA: 1.3 WD (mm): 0.3 60x PlanApo N SC Oil NA: 1.4 WD (mm): 0.12 60x UPlanApo HR Oil Coverglass (mm): 0.13-0.19 NA: 1.50 WD (mm): 0.11
Detectors	High Sensitivity Spectral Detector with 4x GaAsP PMT, 1x Trans PMT
Laser lines (nm)	Solid state: 405 / 445 / 488 / 514 / 561 / 594 / 640
Pinhole size	variable
Spectral separation	Motorized Volume Phase Holographic (VPH) transmission diffraction grating
Hybrid-Scanner	Galvo: 512x512 @ 1 fps (16 fps interlaced mode); Tornado scanning for fast ROI scanning Resonant: 512x512 @ 30 fps; max. speed: 512x32 @ 438 fps
Software	Olympus FV-3000
Filters for fluorescence	Filtersystem (em.-color, dye): excitation \| emission Triple filter cube DAPI/FITC/TRITC (Chroma 69000): DAPI: 392 - 416 nm \| 440 - 462 nm FITC: 473 - 495 nm \| 543 - 561 nm TRITC: 507 - 533 nm \| 574 - 618 nm
UV-VIS illumination	CoolLED pE-300white
Transmitted light	LED (IX3-LHLEDC)
Contrast enhancement	Differentiel interference contrast (DIC)
CO2 controller	Cell vivo CB1G
Temperature controller	Cell vivo CBT with Pecon heating unit 2000
Large incubation chamber	Cell vivo/Pecon
Mini-incubation chamber for PiezoZ	Cell vivo/Pecon
Actively damped optical table	Newport Vision IsoStation

Applications

Confocal, Live imaging, FRET, Photoactivation/-conversion, Spectral unmixing

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