UMIF
UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)
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Abberior MINFLUX
Location: room 01.068, first floor
Excitation laser lines: 405nm, 488nm (cw), 561nm (pulsed), 640nm (pulsed & cw)
MINFLUX laser lines: 640nm
MINFLUX imaging modes: 2D, 3D
Software: Abberior Imspector
Funding: European Regional Development Fund (ERDF)
| Microscope | Olympus IX83 |
| Objectives |
100x UPlanXApo NA: 1.45 WD (mm): 0.13 |
| Detectors | 4xAPD, 1PMT |
| Excitation laser lines [nm] |
Pulsed & continuous wave: 640nm Pulsed: 561nm Continuous wave: 405nm, 488nm |
| MINFLUX laser lines [nm] | 640 |
| Pinhole size | Variable |
| 2D/3D MINFLUX | Localization precision 1-3 nm in XY (2D mode) and in Z (3D mode) |
| Detection channels |
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| Filters for epi-fluorescence |
Filtersystem (em.-color, dye): Quad-band, EX | BS | EM [nm]
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| UV-VIS illumination | CoolLED pE-4000, 16 LEDs for excitation in the range 365-770 nm |
| Halogen lamp | 100W, standard housing |
| Software | Abberior Imspector |
| MINFLUX 3D |
Electronically deformable mirrors for 3D MINFLUX @ 640 nm and localization precision 1-3nm in XYZ |
| Alignment | Motorized auto-alignment of all lasers and pinhole |
| Motorized XY stage | Olympus |
| XYZ stabilization |
Physic Instruments PI Nano XYZ, active sample stabilization with nm precision alongs all 3 dimensions 975nm laser (Cobolt), CCD camera (The Imaging Source) |
| Scanners |
QUADscan beam scanner Ultra fast beam scanner for MINFLUX imaging |
| Actively damped optical table | Thorlabs |