UMIF
UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)
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User #1000
Abberior MINFLUX
Location: Campus Forschung (N27), room 01.068, first floor
Excitation laser lines: 405nm, 488nm (cw), 561 (cw), 561nm (pulsed), 640 (cw), 640nm (pulsed & cw)
MINFLUX laser lines: 561nm, 640nm
MINFLUX imaging modes: 2D, 3D
STED laser line: 775nm
STED imaging modes: 2D, 3D
Software: Abberior Imspector
Funding: European Regional Development Fund (ERDF) under the Operational Programme Hamburg ERDF 2014-2020, REACT-EU, awarded by the Hamburgische Investitions- und Förderbank (IFB). Grant no. 51164232
| Microscope | Olympus IX83 | ||||||
| Objectives |
100x UPlanXApo NA: 1.45 WD (mm): 0.13 |
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| Detectors | 4xAPD, 1PMT | ||||||
| Excitation laser lines [nm] |
Pulsed & continuous wave: 640 Pulsed: 561 Continuous wave: 405, 488, 561, 640 |
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| MINFLUX laser lines [nm] | 561, 640 | ||||||
| STED laser line |
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| Pinhole size | Variable | ||||||
| 2D/3D MINFLUX | Localization precision 1-3 nm in XY (2D mode) and in Z (3D mode) | ||||||
| Gated STED | Resolution <30x30nm in XY | ||||||
| Detection channels |
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| Filters for epi-fluorescence |
Filtersystem (em.-color, dye): Quad-band, EX | BS | EM [nm]
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| UV-VIS illumination | CoolLED pE-4000, 16 LEDs for excitation in the range 365-770 nm | ||||||
| Halogen lamp | 100W, standard housing | ||||||
| Software | Abberior Imspector | ||||||
| MINFLUX 3D |
Electronically deformable mirrors for 3D MINFLUX @ 640 nm and localization precision 1-3nm in XYZ |
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| easy3D STED | Spatial light modulator for 3D STED @ 775 nm and resolution <100x100x100nm in XYZ | ||||||
| Alignment | Motorized auto-alignment of all lasers and pinhole | ||||||
| Motorized XY stage | Olympus | ||||||
| XYZ stabilization |
Physic Instruments PI Nano XYZ, active sample stabilization with nm precision alongs all 3 dimensions 975nm laser (Cobolt), CCD camera (The Imaging Source) |
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| Scanners |
QUADscan beam scanner Ultra fast beam scanner for MINFLUX imaging |
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| Actively damped optical table | Thorlabs |