UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

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User #1000

Olympus Widefield

Location: Rothe-Geussenhainer-Haus (N45), third floor

Fluorescence: DAPI / GFP / RFP / Cy5 / Cy7

Software: Cell Sense Dimension v3

Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

Microscope	Olympus IX-83
Objectives	4x UPLFLN PH NA: 0.13 WD (mm): 17 Pixel size in image (µm): 1.622 20x UCPLFLN PH NA: 0.7 WD (mm): 1.3 Pixel size in image (µm): 0.338 100x UPLXAPO O NA: 1.45 WD (mm): 0.13 Pixel size in image (µm): 0.064
DIC prisms	Condenser: DIC100 Slider: IX3 DICTA
Cameras	Monochrome: Hamamatsu Flash 4.0 LTPlus Color: SC50
Fluorescence filters fluo-turret	Filterset no. (em.-color; dye): excitation \| beamsplitter \| emission Pentaband DAPI/FITC/Cy3/Cy5/Cy7: ex: 352-404/460-488/542-566/626-644/721-749 \| em: 412-450/499-532/578-613/660-703/766-853 DAPI (blue, AF405, DAPI): ex: 371-399 \| BS 409 \| em: 416-480 GFP (green, GFP, AF488): ex: 459-487 \| BS 494 \| em: 500-540 TRITC (red; RFP, AF568): ex: 530-554 \| BS 559 \| em: 570-620 Cy5 (far-red, AF633, CY5): ex: 609-647 \| BS 655 \| 633-703
Fluorescence excitation (LED)	Excitation (nm) Channel 1: 365, 385, 405, 435 nm Channel 2: 460, 470, 490, 500 nm Channel 3: 525, 550, 580, 595 nm Channel 4: 635, 660, 740, 770 nm;
UV-VIS illumination	CoolLED pE-4000, 16 LEDs for excitation in the range 365-770 nm
Halogen lamp	100W 12V, standard housing
Acquisition software	OlympusCell Sense Dimension V 3.1
Incubation unit	Pecon cellVIVO closed large chamber for temp./CO2/humidity
Motorized XY-stage	Märzhäuser Tango
Hardware autofocus	ZDC2
Passively damped optical board	Thorlabs

Widefield