Abbelight SAFe360

Abbelight SAFe360

DFG code: INST 152/933-1


Location: Campus Forschung (N27), room 02.046, second floor

Laser lines: 640/488/405

Software: Abbelight NEO

MicroscopeOlympus IX-83

100x UPlan Apo HR TIRF Oil

NA: 1.50

WD (mm): 0.11 (CS: 0.13-0.19 @ 37 °C)

Pixel size in image (µm): 0.96


2x Hamamatsu ORCA-fusion C14440

Laser lines (nm)


  • SMLM acquisition: 640
  • Widefield imaging, fluorophore reactivation: 488 / 405
Acquisition softwareAbbelight Neo acquisition
Analysis software

Abbelight NEO analysis

Image scanning technologyAdaptative Scanning for Tuneable Excitation Regions (ASTER)
TIRF light path TIRF translational stage for epi, HiLO & TIRF excitation
2D SMLMAbbelight SAFe 180/360, simultaneous dual-color, spectral demixing
3D SMLM extensionAbbelight SAFe 360 with astigmatism lens
Hardware autofocus


Actively damped optical tableNewport Vision IsoStation


2D/3D Multicolor SMLM, Superresolution
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