UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

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Zeiss MP in vivo platform

DFG code: INST 152/876-1 FUGG

Location: Campus Forschung (N27), room 00.027, ground floor

Laser lines: Pulsed: Tunable 680-1300nm, Fixed 1040nm, CW: 405/445/488/561/639nm

Software: ZEN

Click here for a detailed description of the components

Microscope

Zeiss Axio Examiner.Z1 with LSM 980 and Airyscan 2 (upright)

DFG major instrumentation proposal number

INST 152/876-1 FUGG

Objectives

10x Plan-APOCHROMAT

NA: 0.45

WD (=working distance; mm): 2.0

20x Plan-APOCHROMAT

NA: 0.8

WD (mm): 0.55

20x W Plan-APOCHROMAT Corr DIC

Refractive index: 1.33-1.36

NA: 1.0

WD (mm): 2.4

63x Plan-APOCHROMAT Oil DIC

NA: 1.40

WD (mm): 0.19

Detectors

Confocal unit:

- 32x GaAsP

- 2x PMT (UV&NIR)

- Airyscan 2

External:

- 4x NDD-PMT (non-descanned detectors)

Laser lines (nm)

SpectraPhysics InSight X3+	680-1030 (tunable)	1040 (fixed)
Output power	>1.5W at 700nm >2.4W at 800nm >3.0W at 900nm >2.7W at 1000nm >2.4W at 1100nm >2.1W at 1200nm >1.5W at 1300nm	>3.5W
Repetition rate	80MHz +/- 0.5MHz	80MHz +/- 0.5MHz
Pulse width	<120fs	<170fs

CW: 405 / 445 / 488 / 561 / 639nm

Pinhole size

variable

Software

Zeiss ZEN

Filters for fluorescence

For 32+2 channel spectral detection: tunable between 380-900nm

For eyepiece:

Filter system (em.-color, dye): excitation | beamsplitter | emission

49 (blue; AF405, DAPI):
BP 365 | FT 395 | BP 445/50

38 (green; AF488, GFP):
BP 470/40 | FT 495 | BP 525/50

43 (red; AF568, mCherry):
BP 550/25 | FT 570 | BP 605/70

For Airyscan:

BP 420-480 + BP 495-550
BP 420-480 + BP 570-630
BP 420-500 + LP 605
BP 465-505 + BP 525-585
BP 495-550 + BP 570-630
BP 495-560 + LP 660
BP 570-620 + LP 660

For non-descanned detectors (NDD):

Filter system (em.-color, dye): beamsplitter | emission

NDD1 (far-red, AF647, CY5):
LP | BP 645-710 IR+

NDD2 (cyan; CFP):
LP 490 | BP 485 IR+

NDD3 (green, AF488, GFP):
LP 555 | BP 500-550 IR+

NDD4 (red; AF568, mCherry):
mirror | BP 570-610 IR+

UV-VIS illumination

HXP 120V

Halogen lamp

100W 12V

Scanner

Variable
Multiplex: 4 spots or 8 spots with Airyscan

Actively damped optical table

Newport Smart Table UT2

Applications

Confocal, Superresolution, FRET, Widefield, Multiphoton, Spectral unmixing, Tunable excitation, Airyscan

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