UMIF

10x Plan-APOCHROMAT

NA: 0.45

WD (=working distance; mm): 2.0

20x Plan-APOCHROMAT

NA: 0.8

WD (mm): 0.55

20x W Plan-APOCHROMAT Corr DIC

Refractive index: 1.33-1.36

NA: 1.0

WD (mm): 2.4

63x Plan-APOCHROMAT Oil DIC

NA: 1.40

WD (mm): 0.19

Confocal unit:

- 32x GaAsP

- 2x PMT (UV&NIR)

- Airyscan 2

External:

- 4x NDD-PMT (non-descanned detectors)

Pulsed: tunable 680-1300nm, fixed 1040nm (SpectraPhysics InSight X3+)

CW: 405 / 445 / 488 / 561 / 639 nm

For 32+2 channel spectral detection: tunable between 380-900nm

For eyepiece:

Filter system (em.-color, dye): excitation | beamsplitter | emission

• 49 (blue; AF405, DAPI):
  BP 365 | FT 395 | BP 445/50

• 38 (green; AF488, GFP):
  BP 470/40 | FT 495 | BP 525/50

• 43 (red; AF568, mCherry):
  BP 550/25 | FT 570 | BP 605/70

For Airyscan:

1. BP 420-480 + BP 495-550
2. BP 420-480 + BP 570-630
3. BP 420-500 + LP 605
4. BP 465-505 + BP 525-585
5. BP 495-550 + BP 570-630
6. BP 495-560 + LP 660
7. BP 570-620 + LP 660

For non-descanned detectors (NDD):

Filter system (em.-color, dye): beamsplitter | emission 

• NDD1 (far-red, AF647, CY5):
  LP | BP 645-710 IR+

• NDD2 (cyan; CFP):
  LP 490 | BP 485 IR+

• NDD3 (green, AF488, GFP):
  LP 555  | BP 500-550 IR+

• NDD4 (red; AF568, mCherry):
  mirror | BP 570-610 IR+

• Variable
• Multiplex: 4 spots or 8 spots with Airyscan