UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

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Zeiss MP in vivo platform

Zeiss MP in vivo platform

DFG code: INST 152/876-1 FUGG

 

Location: room 00.027, ground floor

Laser lines: Pulsed: Tunable 680-1300nm, Fixed 1040nm, CW: 405/445/488/561/639nm

Software: ZEN

 

Microscope Zeiss Axio Examiner.Z1 with LSM 980 and Airyscan 2 (upright)
DFG major instrumentation proposal number INST 152/876-1 FUGG
Objectives

10x Plan-APOCHROMAT

NA: 0.45

WD (=working distance; mm): 2.0


20x Plan-APOCHROMAT

NA: 0.8

WD (mm): 0.55


20x W Plan-APOCHROMAT Corr DIC

Refractive index: 1.33-1.36

NA: 1.0

WD (mm): 2.4


63x Plan-APOCHROMAT Oil DIC

NA: 1.40

WD (mm): 0.19

Detectors

Confocal unit:

- 32x GaAsP

- 2x PMT (UV&NIR)

- Airyscan 2

External:

- 4x NDD-PMT (non-descanned detectors)

Laser lines (nm)

Pulsed: tunable 680-1300nm, fixed 1040nm (SpectraPhysics InSight X3+)

CW: 405 / 445 / 488 / 561 / 639 nm

Pinhole size variable
Software Zeiss ZEN
Filters for fluorescence

For 32+2 channel spectral detection: tunable between 380-900nm

For eyepiece:

Filter system (em.-color, dye): excitation | beamsplitter | emission

  • 49 (blue; AF405, DAPI):
    BP 365 | FT 395 | BP 445/50
  • 38 (green; AF488, GFP):
    BP 470/40 | FT 495 | BP 525/50
  • 43 (red; AF568, mCherry):
    BP 550/25 | FT 570 | BP 605/70

For Airyscan:

  1. BP 420-480 + BP 495-550
  2. BP 420-480 + BP 570-630
  3. BP 420-500 + LP 605
  4. BP 465-505 + BP 525-585
  5. BP 495-550 + BP 570-630
  6. BP 495-560 + LP 660
  7. BP 570-620 + LP 660

For non-descanned detectors (NDD):

Filter system (em.-color, dye): beamsplitter | emission 

  • NDD1 (far-red, AF647, CY5):
    LP | BP 645-710 IR+
  • NDD2 (cyan; CFP):
    LP 490 | BP 485 IR+
  • NDD3 (green, AF488, GFP):
    LP 555  | BP 500-550 IR+
  • NDD4 (red; AF568, mCherry):
    mirror | BP 570-610 IR+
UV-lamp HXP 120 V
Halogen lamp 100W 12V
Scanner
  • Variable
  • Multiplex: 4 spots or 8 spots with Airyscan
Actively damped optical table Newport Smart Table UT2

 

Applications

Confocal, Superresolution, FRET, Widefield, Multiphoton, Spectral unmixing, Tunable excitation, Airyscan
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