UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)
Location: room 00.027, ground floor
Laser lines: Pulsed: Tunable 680-1300nm, Fixed 1040nm, CW: 405/445/488/561/639nm
Software: ZEN
Microscope | Zeiss Axio Examiner.Z1 with LSM 980 and Airyscan 2 (upright) |
DFG major instrumentation proposal number | INST 152/876-1 FUGG |
Objectives |
10x Plan-APOCHROMAT NA: 0.45 WD (=working distance; mm): 2.0 20x Plan-APOCHROMAT NA: 0.8 WD (mm): 0.55 20x W Plan-APOCHROMAT Corr DIC Refractive index: 1.33-1.36 NA: 1.0 WD (mm): 2.4 63x Plan-APOCHROMAT Oil DIC NA: 1.40 WD (mm): 0.19 |
Detectors |
Confocal unit: - 32x GaAsP - 2x PMT (UV&NIR) - Airyscan 2 External: - 4x NDD-PMT (non-descanned detectors) |
Laser lines (nm) |
Pulsed: tunable 680-1300nm, fixed 1040nm (SpectraPhysics InSight X3+) CW: 405 / 445 / 488 / 561 / 639 nm |
Pinhole size | variable |
Software | Zeiss ZEN |
Filters for fluorescence |
For 32+2 channel spectral detection: tunable between 380-900nm For eyepiece: Filter system (em.-color, dye): excitation | beamsplitter | emission
For Airyscan:
For non-descanned detectors (NDD): Filter system (em.-color, dye): beamsplitter | emission
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UV-lamp | HXP 120 V |
Halogen lamp | 100W 12V |
Scanner |
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Actively damped optical table | Newport Smart Table UT2 |