UMIF
UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)
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Olympus TIRFM
Location: room 02.029, second floor
Laser lines: 405nm/488nm/561nm/640nm
Software: Cell Sense
Live cell imaging: Environmental chamber with temperature, humidity and CO2 control
| Microscope | Olympus IX-81 |
| Objectives |
10x U Plan Semi Apo PH1 NA: 0.30 WD (mm): 10 Pixel size in image (µm): 1.161 20x UPlan FL N NA: 0.50 WD (mm): 2.1 Pixel size in image (µm): 0.580 40x UPlan S Apo NA: 0.90 WD (mm): 0.18 (CS: 0.11-0.23 @ 23 or 37 °C) Pixel size in image (µm): 0.290 60x Plan Apo N TIRF Oil NA: 1.45 WD (mm): 0.1 (CS: 0.13-0.19 @ 37 °C) Pixel size in image (µm): 0.194 100x UPlan Apo HR TIRF Oil NA: 1.50 WD (mm): 0.11 (CS: 0.13-0.19 @ 37 °C) Pixel size in image (µm): 0.116 150x U Apo N TIRF Oil NA: 1.45 WD (mm): 0.07 (CS: 0.13-0.19) Pixel size in image (µm): 0.078 |
| DIC prisms |
Condenser:
Slider:
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Cameras |
Hamamatsu Flash 4.0 V2 Gain: 0.5 e-/ADU |
| Laser lines (nm) |
Solid-state: 405 / 488 / 561 / 640 |
| Fluorescence filters fluo-turret: |
Filterset no. (em.-color; dye): excitation | beamsplitter | emission
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| Fluorescence excitation filters UV-lamp |
Filterset name (ex.-color; dye): excitation (nm)
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UV-Lamp |
75W xenon lamp |
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Halogen lamp |
100W 12V, standard housing |
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Acquisition software |
OlympusCell Sense V1.14 |
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TIRF light path |
Motorized, for 405/488/561/640nm excitable fluorophores |
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Incubation unit |
Evotec closed large chamber for temp./CO2/humidity |
| Hardware autofocus |
ZDC2 |
| Actively damped optical table | Newport |