UMIF
UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)
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User #1000
Abbelight SAFe360
DFG code: INST 152/933-1
Location: Campus Forschung (N27), room 02.046, second floor
Laser lines: 640/488/405
Software: Abbelight NEO
| Microscope | Olympus IX-83 |
| Objectives |
100x UPlan Apo HR TIRF Oil NA: 1.50 WD (mm): 0.11 (CS: 0.13-0.19 @ 37 °C) Pixel size in image (µm): 0.96 |
| Cameras |
2x Hamamatsu ORCA-fusion C14440 |
| Laser lines (nm) |
Solid-state:
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| Acquisition software | Abbelight Neo acquisition |
| Analysis software |
Abbelight NEO analysis |
| Image scanning technology | Adaptative Scanning for Tuneable Excitation Regions (ASTER) |
| TIRF light path | TIRF translational stage for epi, HiLO & TIRF excitation |
| 2D SMLM | Abbelight SAFe 180/360, simultaneous dual-color, spectral demixing |
| 3D SMLM extension | Abbelight SAFe 360 with astigmatism lens |
| Hardware autofocus |
IX3-ZDC |
| Actively damped optical table | Newport Vision IsoStation |