UMIF
UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)
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User #1000
Aurox SD
Location: Campus Forschung (N27), room 02.053, second floor
Fluorescence: DAPI / GFP / RFP / Far-Red
Software: Visionary
Live cell imaging: Environmental chamber with temperature, humidity and CO2 control
| Microscope | Zeiss Axiovert 200M |
| Objectives |
10x EC Plan-Neofluar NA: 0.3 WD (mm): 5.2 Pixel size in image (µm): 0.65 20x EC Plan-Neofluar NA: 0.5 WD (mm): 2.0 Pixel size in image (µm): 0.325 10x EC Plan-Neofluar Ph1 NA: 0.3 WD (mm): 5.2 Pixel size in image (µm): 0.65 20x EC Plan-Neofluar Ph2 NA: 0.5 WD (mm): 2.0 Pixel size in image (µm): 0.325 63x Plan-Apochromat Ph3 Oil NA: 1.4 WD (mm): 0.19 Pixel size in image (µm): 0.103 |
| Camera | 1x Photometrics BSI Express |
| Spinning Disk unit | Aurox Clarity |
| Emission filters spinning disk (for image acquisition) |
blue (DAPI): Ex 392/23, Em 447/60 |
| Fluorescence filters in microscope stand |
Filtersystem (em.-color, dye): excitation | beamsplitter | emission
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| UV-VIS illumination |
For visual inspection: EXFO X-cite 120 For imaging: CoolLED pE-4000, 16 LEDs for excitation in the range 365-770 nm |
| Halogen lamp | 100W, standard housing |
| Software | Visionary |
| Incubation unit | Solent Scientific |
| Piezo focus drive | Ludl Electronic Products PiezoZ, 200µm travel range |
| Motorized XY stage |
Ludl Electronic Products BioPrecision |
| Actively damped optical table | Newport |