UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

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User #1000

Leica TCS SP8 X

Location: Campus Forschung (N27), room 02.053, second floor

Laser lines: WLL 470-670 nm; Diode 405 nm; Multi-Ar 458 nm/476 nm/488 nm/496 nm/514 nm; DPSS 561 nm; HeNe 594nm, HeNe 633 nm

Software: Leica LAS X

Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

Microscope	Leica DMi8
Objectives	10x HC PL APO CS NA: 0.40 WD (=working distance; mm): 2.2 20x HC PL APO IMM/CORR CS2 NA: 0.75 WD (mm): 0.67 (W) 25x WATER HC FLUOTAR L VISIR NA: 0.95 WD (mm): 2.5 40x HC PL APO W motCORR CS2 Coverglass (mm): 0.14-0.18 NA: 1.10 WD (mm): 0.65 63x HC PL APO Oil CS2 NA: 1.40 WD (mm): 0.14
Detectors	3x HyD, 2x PMT, 1x Trans-PMT
Laser lines (nm)	White light laser, pulsed (WLL): 470-670 Diode: 405 Multi-Ar: 458 / 476 / 488 / 496 / 514 DPSS: 561 HeNe: 594 / 633
Pinhole size	variable
Spectral separation	AOBS® + spectral detectors
Software	Leica LAS X SP8 v. 3.5.2
Filters for fluorescence	Filtersystem (em.-color, dye): excitation \| beamsplitter \| emission L5 ET (green; AF488, GFP): BP 480/40 \| FP 505 \| em. BP 527/30 A (blue; DAPI): BP 340-380 \| FP \| LP 425 I 3 (green; AF488, GFP): BP 450-490 \| FP \| LP 515 N 2.1 (red; AF568, mCherry): BP 515-560 \| FP \| LP 590
UV-VIS illumination	EL 6000 120W (LQHXP 120 LEJ)
Halogen lamp	100W 12V
CO2 controller	BRICK/Ludin
Temperature controller	Ludin Cube
Piezo focus drive	SuperZ Galvo type H (travel range: 1500 µm; reproducibility: 40 nm)
Tandem-Scanner	Variable: 512x512 @ 7 fps; max. 8192x8192 px; max. 3600 lines/s; 1x-48x zoom Resonant: 512x512 @ 40 fps; max. 832x832 px; max. 24,000 lines/s; 2x-24x zoom
Large incubation chamber	Ludin
Mini-incubation chamber for PiezoZ	Ludin
Actively damped optical table	Newport

Confocal, Live imaging, FRET, FRAP, Photoactivation/-conversion, Spectral unmixing, Tunable excitation, gated detection