UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

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User #1000

Visitron SD-TIRF

Location: Campus Forschung (N27), room 02.053, second floor

Laser lines: 405nm/445nm/488nm/515nm/561nm/640nm

Software: VisiView

Live cell Imaging: Add-on environmental chamber with temperature, humidity and CO2 control

Click here for a detailed description of the components

Microscope	Nikon Eclipse TiE
Objectives	10x CFI Plan Fluor DL Phase NA: 0.30 WD (mm): 15.2 Pixel size in image (µm): 1.100 20x CFI Plan Fluor DLL Phase NA: 0.51 WD (mm): 2.1 Pixel size in image (µm): 0.550 40x CFI Plan Fluor Oil NA: 1.30 WD (mm): 0.2 Pixel size in image (µm): 0.275 60x Apo TIRF (corr.) Oil NA: 1.49 WD (mm): 0.13 (CS: 0.10-0.22 @ 23 or 37 °C) Pixel size in image (µm): SpinningDisk: 0.183; SORA disk: 0.065 100x CFI Plan Apo Lambda NA: 1.45 WD (mm): 0.13 Pixel size in image (µm): spinningDisk: 0.110; SORA disk: 0.039
Cameras	2x Photometrics Prime 95B (back-illuminated sCMOS, 11µm pixel-size, 1200x1200 pixels)
Laser lines (nm)	Solid-state: 405 / 445 / 488 / 515 / 561 / 640
Spinning Disk unit	Yokogawa CSU W-1 SoRa in dual-camera configuration 1) Confocal disk (50µm pinholes) 2) SoRa disk for super-resolution
Emission filters spinning disk (for laser-related fluorescence)	Camera 1: DAPI, ET460 (W50) CFP ET470 (W24) GFP, ET525 (W50) Camera 2: YFP ET535 (W30) mCherry, ET609 (W54) CY5 ET700 (W75)
Dichroic in spinning disk unit	405/488/561/640 (used with the respective channels) 445/515/640 (used for 445, 515 and 640 channels)
Dichroic for dual camera mode	561LP 514LP
Fluorescence filters in microscope stand	Filtersystem (em.-color, dye): excitation \| beamsplitter \| emission DAPI (blue; DAPI): BP 325-375 \| DM 400 \| BP 435-485 GFP (green; AF488, GFP): BP 450-490 \| DM 495 \| BP 500-550 TxRed (red; AF568, mCherry): BP 540-580 \| DM 585 \| BP 593-668
Dichroic mirrors in microscope stand for TIRF and FRAP	TIRF-FRAP1: 405/488/561/640 rpc BS (Chroma; 2mm) TIRF-FRAP2: 442/514 rpc BS (Chroma; 2mm)
UV-VIS illumination	SOLA-SM Light Engines, white light LED, 380-680nm
Transmitted light lamp	precisExcite, High-Power 525nm LED
Software	VisiView
Incubation unit	okolab bold line
FRAP-TIRF unit	Roper iLAS2 for FRAP&TIRF with all laser lines
Piezo focus drive	Ludl NanoPrecision PiezoZ, 350µm travel range
Motorized XY stage	Ludl BioPrecision3 LM
Auto-focus	Nikon PerfectFocus system
Actively damped optical table	Newport

Applications

SpinningDisk, TIRF, Superresolution, Live imaging, FRET, Widefield, FRAP, Photoactivation/-conversion

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