UMIF
UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)
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User #1000
Abberior STED
Location: Campus Forschung (N27), room 02.046, second floor
Excitation laser lines: 405, 518nm, 640nm (pulsed & cw), 488nm, 561nm (pulsed)
STED laser lines: 595nm, 775nm
STED imaging modes: gated-STED, RESCue, DyMIN, MINFIELD
Software: Abberior Imspector
Live cell imaging: Add-on environmental perfusion chamber with temperature, humidity and CO2 control
STED publications @ UMIF: [DOCX]
| Microscope | Nikon Ti-E with perfect focus system | ||||||||||||
| Objectives |
60x P-Apo Oil NA: 1.40 WD (mm): 0.13 |
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| Detectors | 4xAPD, 1xCCD camera (The Imaging Source DMK 33G274) | ||||||||||||
| Excitation laser lines [nm] |
Pulsed & continuous wave: 518 / 640 Pulsed: 405 / 488 / 561
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| STED laser lines |
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| Pinhole size | Variable | ||||||||||||
| Gated STED | Resolution <30x30nm in XY | ||||||||||||
| Detection channels |
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| Filters for epi-fluorescence |
Filtersystem (em.-color, dye): excitation
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| UV-VIS illumination | CoolLED pE-2, 4 LEDs: 400, 490, 565nm, 635nm | ||||||||||||
| Halogen lamp | 100W | ||||||||||||
| CO2 controller | Okolab | ||||||||||||
| Temperature controller | Okolab | ||||||||||||
| Software | Abberior Imspector | ||||||||||||
| easy 3D |
Spatial light modulators for 3D STED @ 595 & 775 nm and resolution <100x100x100nm in XYZ |
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| RESCue STED | for bleaching reduction | ||||||||||||
| DyMIN STED | Light dose management for super-resolution live imaging | ||||||||||||
| MINFIELD STED | Light dose management for higher contrast and resolution | ||||||||||||
| Motorized XY stage | Nikon | ||||||||||||
| Piezo focus drive | Mad City Labs | ||||||||||||
| Quad-Scanner | 512x512 @ 5 fps; max. 16000x16000 px | ||||||||||||
| FLIM/FCS/FCCS | Becker&Hickl SPC150 TCSPC board | ||||||||||||
| Actively damped optical table | Thorlabs |