UMIF
UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)
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Abberior STED
Location: room 02.046, second floor
Excitation laser lines: 405, 518nm, 640nm (pulsed & cw), 488nm, 561nm (pulsed)
STED laser lines: 595nm, 775nm
STED imaging modes: gated-STED, RESCue, DyMIN, MINFIELD
Software: Abberior Imspector
Live cell imaging: Add-on environmental perfusion chamber with temperature, humidity and CO2 control
| Microscope | Nikon Ti-E with perfect focus system |
| Objectives |
60x P-Apo Oil NA: 1.40 WD (mm): 0.13 |
| Detectors | 4xAPD, 1xCCD camera (The Imaging Source DMK 33G274) |
| Excitation laser lines [nm] |
Pulsed & continuous wave: 518 / 640 Pulsed: 405 / 488 / 561
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| STED laser lines [nm] | 595 / 775 |
| Pinhole size | Variable |
| Gated STED | Resolution <30x30nm in XY |
| Detection channels |
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| Filters for epi-fluorescence |
Filtersystem (em.-color, dye): excitation
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| UV-VIS illumination | LED |
| Halogen lamp | 100W |
| CO2 controller | Okolab |
| Temperature controller | Okolab |
| Software | Abberior Imspector |
| easy 3D |
Spatial light modulators for 3D STED @ 595 & 775 nm and resolution <100x100x100nm in XYZ |
| RESCue STED | for bleaching reduction |
| DyMIN STED | Light dose management for super-resolution live imaging |
| MINFIELD STED | Light dose management for higher contrast and resolution |
| Motorized XY stage | M-687 PILine (Physik Instrumente) |
| Piezo focus drive | P-736 PInano (Physik Instrumente, 220μm travel range) |
| Quad-Scanner | 512x512 @ 5 fps; max. 16000x16000 px |
| FLIM/FCS/FCCS | Becker&Hickl SPC150 TCSPC board |
| Actively damped optical table | Thorlabs |