UKE Microscopy Imaging Facility (UMIF)

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

MINFLUX SARS-CoV2

MINFLUX microscopy made the visualization of SARS-CoV2 virus molecules possible.

(1) Confocal, (2) MINFLUX, (3) MINFLUX superimposed with EM,

(4) schematic overlay with SARS-CoV2 model

CONFOCAL MICROSCOPY III

GFAP (for astrocytes) / Iba-1 (microglia) / Hoechst (nucleus).

Author: Prof Saez

CONFOCAL MICROSCOPY I

Optical nerve

Tiled scan

CONFOCAL MICROSCOPY II

Heart tissue section

SUPER-RESOLUTION IMAGE OF DETYROSINATED TUBULIN

Detyrosinated tubulin in neurons.

left = confocal, right = STED

Super-resolution 2D dSTORM image of nuclear pore complex (NPC)

NPC with Abberior FLUX640

STRUCTURED ILLUMINATION MICROSCOPY (SIM)

Multicolor labeling of an eukaryotic cell.

red =actin, green = focal adhesions, blue = DAPI

COMBINED SD-TIRF IMAGING OF CELLULAR VESICLES

3D reconstruction of CLN3-vesicles and lysosomes in HeLa cells.

green = CLN3-GFP, red = lysosomes

SUPER-RESOLUTION RADIAL FLUORESCENCE MICROSCOPY (SRRF)

Multi-color HeLa cells

green = F-Actin, red = alpha-Tubulin, gray = TOMM-20

Contact us

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User regulations, booking rules, charging scheme, course material, software, protocols and more

Latest news and events

Urgent Facility movement and your Imaging Plan follow up

Dear users,

I confirm you that the facility movement will start officially 17.11.2025.

Additional to the previous given information starting from 24.11 STED and MINFLUX microscopes will be not available due to their movement.

I cannot predict when these systems will be available again in CF2 but I will let you know asap as I know.

There are some new important points

I need to grant you access to all the new facility rooms (and old one that keeps) naturally after have you been introduced again about the GMO/ laser general instructions.

To do this no matter if you are registered since few days or 15 years you need to re-register

Re-register  is easy and it will be possible till 01.12.2025

To re-register use this link

 UMIF Re-registration Form 2025

The account of users that are not re-registered will be deactivated.

Re-activation of accounts deactivated after 01.12.2025 will occurs once every 3 weeks (because this is a long process that can not prevent UMIF to provide other services).

In order to run as smooth as possible the UMIF movement we count on your understanding and collaboration.

Additionally to the users that have used the Zeiss MP for acquiring data for their Thesis  Bachelor PhD or any other thesis could you help me during this week to fill up this table?

 No.  Type of degree  Author(s), title  Year of degree  URL/DOI
 1.        
 2.        
 3.        
 ...        

 

best wishes

Antonio Virgilio Failla

Shedding light on pathogens using MINFLUX nanoscopy

Dear all,

After a kind of long pause UMIF re-start again to contribute in the development of bio-imaging proof of principle experiments.

A special thanks to all the co-authors for their valuable help and contribution.

I need to add some more words: this work would not exist without the key role Dr. Carsten had in designing the experiments and writing the manuscript and Prof. Aepfelbacher  in pushing and motivating us to go further.

Please get a look  at the paper:

https://analyticalscience.wiley.com/content/article-do/shedding-light-pathogens-using-minflux-nanoscopy

and get inspired!!!

We are looking forward to support you in discover your biological nanostructures on the molecular level!!!

best wishes

Antonio Virgilio Failla

We are Recruiting

We are Recruiting.


We at university medical centre Hamburg Eppendorf are searching for a motivated, enthusiastic, ambitious and with the feeling of purpose light microscopy imaging specialist.


If you would like to know more about the UKE Microscopy Imaging Facility (UMIF), please browse www.umif.de.


For further information about the position please do not hesitate to contact me.


If you want to know more and apply for the post please use this link:
https://uke.jobs/index.php?ac=jobad&id=4997


The job advisement is in German but I can provide, on demand, the English version.

Looking forward to your applications.

 

Best Wishes,

Antonio Virgilio Failla

UMIF office has relocated

Dear UMIF users,

Please be informed that the UMIF office has relocated. Our new office is situated in room EG 00.19 N27 on the ground floor.

For those familiar with the building, we are conveniently located right next to seminar room 14.

We welcome you to visit us at our new location.

Best wishes,

Antonio Virgilio Failla

UMIF Lecture 2024

UMIF together with UKE scientific community is organizing the second UKE  “microscopy from single molecule to living embryo”. This course is the follow up of the first UKE “light microscopy from single molecules to living embryo”. As you can see the word “light” has been removed because this year also Electron microscopy for biomedical application will be covered. Other added topics are: sample preparation for basic advanced and super resolution microscopy. This course will run between November and January and will consist of 17 lectures for a total of 34 hours (that will provide you didactic credits). The course is made for everyone who want to learn about microscopy from the basic concepts to the more advanced super resolution techniques.  In this course will be stressed out how important is the design of a light microscopy experiments and the huge impact that sample preparation has on their successful outcome. Last but not least some basic notion of image analysis and quantification will also be offered. If you are interested in widen your horizon including microscopy in your knowledge portfolio this course is made for you, especially if you would like to get inspired regarding which images techniques are available at UKE and how they could impact your research projects.

Here is the detailed program of the course

Here is the course timeline

Here is the link for the registration

 

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