UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

Olympus-Abbelight SMLM

Olympus-Abbelight SMLM

 

Location: room 01.068, first floor

Laser lines: 405nm/488nm/561nm/640nm

Software: Cell Sense

Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

 

 

Microscope Olympus IX-81
Objectives

100x UPlan Apo HR TIRF Oil

NA: 1.50

WD (mm): 0.11 (CS: 0.13-0.19 @ 37 °C)

Pixel size in image (µm): 0.116


150x U Apo N TIRF Oil

NA: 1.45

WD (mm): 0.07 (CS: 0.13-0.19)

Pixel size in image (µm): 0.078

Cameras

Hamamatsu Flash 4.0 V2

Gain: 0.5 e-/ADU

Laser lines (nm)

Solid-state: 

  • 405 (100mW)
  • 488 (200mW)
  • 561 (100mW)
  • 640 (140mW)
Acquisition software OlympusCell Sense V1.14, Abbelight Neo
Analysis software

Abbelight Neo

TIRF light path  Motorized, for 405/488/561/640 nm excitable fluorophores
3D SMLM extension Abbelight SAFe 180 with astigmatism lens
Hardware autofocus

ZDC2

Actively damped optical table Newport

Applications

Superresolution, 2D/3D Multicolor PALM/STORM
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