UMIF
UKE Microscopy Imaging Facility
Olympus SMLM
Location: room 01.068, first floor
Laser lines: 405nm/488nm/561nm/640nm
Software: Cell Sense
Live cell imaging: Environmental chamber with temperature, humidity and CO2 control
| Microscope | Olympus IX-81 |
| Objectives |
100x UPlan Apo HR TIRF Oil NA: 1.50 WD (mm): 0.11 (CS: 0.13-0.19 @ 37 °C) Pixel size in image (µm): 0.116 150x U Apo N TIRF Oil NA: 1.45 WD (mm): 0.07 (CS: 0.13-0.19) Pixel size in image (µm): 0.078 |
| Cameras |
Hamamatsu Flash 4.0 V2 Gain: 0.5 e-/ADU |
| Laser lines (nm) |
Solid-state:
|
| Acquisition software | OlympusCell Sense V1.14, Abbelight Neo |
| Analysis software |
Abbelight Neo |
| TIRF light path | Motorized, for 405/488/561/640 nm excitable fluorophores |
| 3D SMLM extension | Abbelight SAFe 180 with astigmatism lens |
| Hardware autofocus |
ZDC2 |
| Actively damped optical table | Newport |