UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

Zeiss ApoTome

Zeiss ApoTome

 

Location: room 02.053, second floor

Fluorescence: DAPI / GFP / RFP / Far-Red

Software: Axiovision 4.8.2

Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

 

 

Microscope Zeiss Axiovert 200M
Objectives (installed)

5x EC Plan-Neofluar

NA: 0.16

WD (mm): 18.5

Pixel size in image (µm): 1.290


10x A-Plan Ph1

NA: 0.25

WD (mm): 4.5

Pixel size in image (µm): 0.645


20x LD Plan-Neofluar Korr Ph2

NA: 0.4

WD (mm): 7.9 – 0.75

Pixel size in image (µm): 0.323


20x Plan-Apochromat

NA: 0.8

WD (mm): 0.55

Pixel size in image (µm): 0.323


40x EC Plan-Apochromat

NA: 1.4

WD (mm): 0.13

Pixel size in image (µm): 0.161


63x Plan-Apochromat Oil DIC

NA: 1.4

WD (mm): 0.19

Pixel size in image (µm): 0.102

Objectives (spare/'Ersatz')

40x EC Plan-Neofluar

NA: 0.75

WD (mm): 0.71

Pixel size in image (µm): 0.161


63x EC Plan-Neofluar Oil

NA: 1.25

WD (mm): 0.10

Pixel size in image (µm): 0.102

DIC-Prisms

Condenser:

  • DIC II and DIC III

Sliders:

  • A: 20x
  • 63x ‘standard’: Oil NA 1.25 and water NA 1.2 (both with DIC III)
  • 63x ‘high contrast’: Oil NA 1.25 and water NA 1.2 (both with DIC II)
Cameras
  • CCD AxioCam HRm 1.4MP monochrome
    • Gain: 0.5 e-/ADU
  • CCD AxioCam MRc 1.4MP color
UV-Lamp

HBO 100W with Hg lamp

Fluorescence filters in microscope stand

Filtersystem (em.-color, dye): excitation | beamsplitter | emission

  • 49 (blue; DAPI):
    G 365 | FT 395 | BP 445/50

  • 44 (green; AF488, GFP):
    BP 475/40 | FT 500 | BP 530/50

  • 43 (red; AF568, mCherry):
    BP 545/25 | FT 570 | BP 605/70

  • 50 (far-red; AF633, CY5):
    BP 640/30 | FT 660 | BP 690/50

  • 32 (optional; far-red; AF680, CY5.5):
    BP 665/45 | FT 695 | BP 725/50

Halogen lamp 100W, standard housing
Software Zeiss Axiovision v4.8.2 32bit (for regular use)
Zeiss Axiovision v4.9.1 SP2 64bit (for very large MosaiX scans)
Incubation unit Pecon XLS1
ApoTome for structured Illumination (VH and VL grids)  
Anti-vibrational plate  Newport

Date

31 March 2017

Applications

Live imaging, Structured illumination, Widefield
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