UMIF

UKE Microscopy Imaging Facility

Improvision SD

Improvision SD

 

Location: room 02.053, second floor

Laser lines: 405nm/488 nm/561 nm

Software: Volocity 6

Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

 

 

Microscope Zeiss Axiovert 200M
Objectives

10x EC Plan-Neofluar

NA: 0.3

WD (mm): 5.2


20x EC Plan-Neofluar

NA: 0.5

WD (mm): 2.0


10x EC Plan-Neofluar Ph1

NA: 0.3

WD (mm): 5.2


20x EC Plan-Neofluar Ph2

NA: 0.5

WD (mm): 2.0


63x Plan-Apochromat Ph3 Oil

NA: 1.4

WD (mm): 0.19

Cameras 1x Hamamatsu EM-CCD C9100-02
Laser lines (nm)

Solid-state: 405 / 488 / 561

Spinning Disk unit Yokogawa CSU-22
Emission filters spinning disk (for laser-related fluorescence)

green, ET 525/50
blue, 452/45
red, ET 590/50
green+red

Dichroic in spinning disk unit

405/488/561/640

Fluorescence filters in microscope stand

Filtersystem (em.-color, dye): excitation | beamsplitter | emission

  • 49 (blue; DAPI):
    G 365 | FT 395 | BP 445/50

  • 09 (green; AF488, GFP):
    BP 450-490 | FT 510 | LP 515

  • 43 (red; AF568, mCherry):
    BP 545/25 | FT 570 | BP 605/70

UV-lamp EXFO X-Cite 120 Series, 120W
Halogen lamp 100W, standard housing
Software Volocity 6
Incubation unit Solent Scientific
FRAP unit PerkinElmer PhotoKinesis
Piezo focus drive Ludl Electronic Products PiezoZ, 200µm travel range
Motorized XY stage

Ludl Electronic Products BioPrecision

Actively damped optical table  

Applications

SpinningDisk, Live imaging, FRET, Widefield, FRAP, Photoactivation/-conversion
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