UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

Leica TCS SP8 X

Leica TCS SP8 X

 

Location: room 02.053, second floor

Laser lines: WLL 470-670 nm; Diode 405 nm; Multi-Ar 458 nm/476 nm/488 nm/496 nm/514 nm; DPSS 561 nm; HeNe 594nm, HeNe 633 nm

Software: Leica LAS X

Live cell imaging: Add-on environmental perfusion chamber with temperature, humidity and CO2 control

 

 

Microscope Leica DMi8
Objectives

10x HC PL APO CS

NA: 0.40

WD (=working distance; mm): 2.2


20x HC PL APO IMM/CORR CS2

NA: 0.75

WD (mm): 0.67 (W)


40x HC PL APO W motCORR CS2

Coverglass (mm): 0.14-0.18

NA: 1.10

WD (mm): 0.65


63x HC PL APO Oil CS2

NA: 1.40

WD (mm): 0.14

Detectors 3x HyD, 2x PMT, 1x Trans-PMT
Laser lines (nm)

White light laser, pulsed (WLL): 470-670

Diode: 405

Multi-Ar: 458 / 476 / 488 / 496 / 514

DPSS: 561

HeNe: 594 / 633

Pinhole size variable
Spectral separation AOBS® + spectral detectors
Software Leica LAS X SP8
Filters for fluorescence

Filtersystem (em.-color, dye): excitation | beamsplitter | emission

  • L5 ET (green; AF488, GFP):
    BP 480/40 | FP 505 | em. BP 527/30
  • A (blue; DAPI):
    BP 340-380 | FP | LP 425
  • I 3 (green; AF488, GFP):
    BP 450-490 | FP | LP 515
  • N 2.1 (red; AF568, mCherry):
    BP 515-560 | FP | LP 590
UV-lamp EL 6000 120W (LQHXP 120 LEJ)
Halogen lamp 100W 12V
CO2 controller BRICK/Ludin
Temperature controller Ludin Cube
Piezo focus drive SuperZ Galvo type H (travel range: 1500 µm; reproducibility: 40 nm)
Tandem-Scanner
  • Variable: 512x512 @ 7 fps; max. 8192x8192 px; max. 3600 lines/s; 1x-48x zoom
  • Resonant: 512x512 @ 40 fps; max. 832x832 px; max. 24,000 lines/s; 2x-24x zoom
Large incubation chamber Ludin
Mini-incubation chamber for PiezoZ Ludin
Actively damped optical table Newport

Applications

Confocal, Live imaging, FRET, FRAP, Photoactivation/-conversion, Spectral unmixing, Tunable excitation, gated detection
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