UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

Leica TCS SP5

Leica TCS SP5

 

Location: room 02.046, second floor

Laser lines: Diode: 405 nm; Multi-Ar 458 nm/476 nm/488 nm/496 nm/514 nm; DPSS 561 nm; HeNe 633 nm

Software: Leica LAS

Live cell imaging: Add-on environmental perfusion chamber with temperature, humidity and CO2 control

 

 

Microscope Leica DMI 6000
Objectives

10x HCPL FLUOTAR

NA: 0.30

WD (=working distance; mm): 11.0


20x HC PL APO CS IMM/CORR 

NA: 0.70

WD (mm): 0.59 (W), =.17 (oil)


25x WATER HC FLUOTAR L VISIR

NA: 0.95

WD (mm): 2.5


40x HC PL APO CS2 Oil

NA: 1.30

WD (mm): 0.24


63x HCX PL APO Lbd. Bl. Oil

NA:  1.4-0.60

WD (mm): 0.1


63x WATER HCX PL APO CS

NA:  1.2

WD (mm): 0.22

Detectors 3x HyD, 1x PMT, 1x Trans-PMT
Laser lines (nm)

Diode: 405

Multi-Ar: 458 / 476 / 488 / 496 / 514

DPSS: 561

HeNe: 633

Pinhole size variable
Spectral separation AOBS® + spectral detectors
Software Leica LAS AF
Filters for fluorescence

Filtersystem (em.-color, dye): excitation | beamsplitter | emission

  • L5 ET (green; AF488, GFP):
    BP 480/40 | FP 505 | em. BP 527/30
  • N3 ET (red; AF568, mCherry):
    BP 546/12 | FP 565 | em. 600/40
  • A (blue; DAPI):
    BP 340-380 | FP | LP 425
  • I 3 (green; AF488, GFP):
    BP 450-490 | FP | LP 515
  • N 2.1 (red; AF568, mCherry):
    BP 515-560 | FP | LP 590
UV-lamp EL 6000 120W (LQHXP 120 LEJ)
Halogen lamp 100W 12V
CO2 controller BRICK/Ludin
Temperature controller Ludin Cube
Piezo focus drive SuperZ Galvo type H (travel range: 500 µm; reproducibility: 40 nm)
Tandem-Scanner
  • Variable: 512x512 @ 5 fps; max. 8192x8192 px; max. 2800 lines/s; 1x-32x zoom
  • Resonant: 512x512 @ 25 fps; max. 1024x1024 px; max. 16.000 lines/s; 1.7x-32x zoom
Large incubation chamber Ludin
Mini-incubation chamber for PiezoZ Ludin
Actively damped optical table Newport 
Silencer for Multi-Ar laser  

 

 

 

 

 

Applications

Confocal, FCS/FCCS, Live imaging, FRET, FRAP, Photoactivation/-conversion, Spectral unmixing
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