UMIF

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

Olympus Widefield

Olympus Widefield

 

Location: Rothe-Geussenhainer-Haus (N45), third floor

Fluorescence: DAPI / GFP / RFP / Cy5 / Cy7

Software: Cell Sense Dimension v3

Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

 

 

Microscope Olympus IX-83
Objectives

4x UPLFLN PH

NA: 0.13

WD (mm): 17

Pixel size in image (µm): 1.622


20x UCPLFLN PH

NA: 0.7

WD (mm): 1.3

Pixel size in image (µm): 0.338


100x UPLXAPO O

NA: 1.45

WD (mm): 0.13

Pixel size in image (µm): 0.064

DIC prisms

Condenser:

  • DIC100

Slider:

  • IX3 DICTA

Cameras

Monochrome: Hamamatsu Flash 4.0 LTPlus

Color: SC50

Fluorescence filters fluo-turret

Filterset no. (em.-color; dye): excitation | beamsplitter | emission

  • Pentaband DAPI/FITC/Cy3/Cy5/Cy7: ex: 352-404/460-488/542-566/626-644/721-749 | em: 412-450/499-532/578-613/660-703/766-853
  • DAPI (blue, AF405, DAPI): ex: 371-399 | BS 409 | em: 416-480
  • GFP (green, GFP, AF488): ex: 459-487 | BS 494 | em: 500-540
  • TRITC (red; RFP, AF568): ex: 530-554 | BS 559 | em: 570-620
  • Cy5 (far-red, AF633, CY5): ex: 609-647 | BS 655 | 633-703
Fluorescence excitation (LED)

Excitation (nm)

  • Channel 1: 365, 385, 405, 435 nm
  • Channel 2: 460, 470, 490, 500 nm
  • Channel 3: 525, 550, 580, 595 nm
  • Channel 4: 635, 660, 740, 770 nm;

UV-VIS illumination

coolLED pE-4000

Halogen lamp

100W 12V, standard housing

Acquisition software

OlympusCell Sense Dimension V 3.1

Incubation unit

Pecon cellVIVO closed large chamber for temp./CO2/humidity
Motorized XY-stage Märzhäuser Tango
Hardware autofocus

ZDC2

Passively damped optical board Thorlabs

Applications

Widefield
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